Genetics Problem Spaces

Introduction Problem Spaces Behavioral Genetics Overview Intro Exercise Research Questions Literature Resources Assignments Student Projects

Behavioral Genetics Research Questions Question I In the introductory exercise, we explored the complex behavior of C. elegans in response to benzaldehyde (Nuttley et al, 2001).  After exposure to high concentrations of benzaldehyde, the nematode develops a negative chemotaxic behavior to the compound.  Nuttley speculates that exposure to high concentrations of benzaldehyde stimulates an aversive response to the compound.  Once the worm acquires this aversive response, it will move away from even low concentrations of benzaldehyde. Is this increase in aversive response a form of non-associative learning or of associative learning?  (Hint: we have stocks of worms that are defective in associative learning and non-associative learning.)  Question II About 40% of the genes of C. elegans have counterparts in vertebrates.  Insight into the roles of these genes in vertebrates can be obtained by studying the genes in nematodes.   An example of a vertebrate protein that can be studied in nematodes in the HSP110.  Dr. Easton and colleagues have determined part of the structure of HSP110 and have characterized its in vitro activity. One clue to its function is that in mammals it is very abundant in brain tissue.  It is interesting to speculate that the protein may be necessary for neuron function.  We have an RNAi vector for reducing expression of the hsp110 gene in C. elegans.  Can this vector be used to test the hypothesis that HSP110 is required for proper neuron function?  (Hint: chemotaxis and learning are examples of neuron mediated activity) Question III Many researchers studying nematode chemotaxis speculate that positive chemotaxis is adaptive because it helps the nematode to locate and move towards its bacterial food.  Growing bacteria release a number of volatile and soluble compounds.  It has been proposed that nematodes have evolved a positive chemotaxis response to these compounds. Can the chemotaxis deficient worms be used to test the hypothesis that chemotaxis allows nematodes to locate and move toward bacteria?