Buffalo State Biology Department C. elegans Project

 
Integration of Undergraduate Laboratory Experience by Use of a Common Experimental Organism: C. elegans
PI: Douglas  Easton Co-PI: Gregory Wadsworth

NSF CCLI-AI track Proposal Sections (pdf files)
         Project Summary
         Project Description
         Citations

Laboratory Handouts for Bio 314 Advanced Cell Biology ( Unfolded Protein Response Project)

         Lab 1 Working with Worms

        Lab 2 Introduction to the Project

        Powerpoint Lecture - Introduction to the Unfolded Protein Response

        Lab 3 Role of UPR in Surviving ER Stress

       Lab 4 Isolation of Total RNA from C. elegans

       Power Point Lecture the Use of RT-PCR to Amplify XBP-1 mRNA

        Lab 5 Cleanup and Electrophoresis of C. elegans RNA

        Lab 6 RT PCR Primer Design for XBP-1 RNA

            Primers for XBP-1

        Lab 7 RT PCR Protocol

        Lab 8 Electropphoresis of RT PCR Product Carried out as in lab 5

        Student Powerpoint Presentation of Project Results

Laboratory Handouts for Zoology and Genetics Labs

        Behavioral Genetics

        Nematodes and Chemotaxis

Laboratory Handout For Introductory Cell Biology

        Gene Expression in C. elegans

Laboratory Handouts for Advanced Cell Biology Using GFP as a Model Protein
for Bioinformatics and Gene Cloning

  Introduction to Online Sequence Databases

    Sequence Analysis Protein Structure-Function Relationships

    Purification of GFP from Bacteria Transformed With the PGLO™ Plasmid*

Modified from instructions in BioRad PGLO GFP kit

Supported by NSF DUE CCLI-AI track