BIO450

Weekly Schedule (Check Frequently for Updates)

            Week 1

            Week 2

            Week 3

            Week 4

            Week 5

            Week 6

            Week 7

            Week 8

            Week 9

 

Course Policies

            Course Policies

            Experimental Flow Diagram

Laboratory Notebooks

Rules for Solutions

 

Class Info

Gene Assignments

PCR Primers

 

Protocols

Initial Solutions and Media

Gene Sequence Analysis

DNA Lysate Prep

Primer Design

PCR Initial Protocol

Plasmid DNA Isolation

Quick Compentent Cells

 

Lectures

            PCR Basics

            DNA Cloning

            Restriction Enzymes

            Insert Orientation

 

General Links

            Biology Workbench

            Wormbase

            NCBI

            NEB restriction enzyme resources

 

PCR Links

            Dolan DNA Learning Center Animation

            NCBI Books: Cell and Molecular Biology Chapter 7.7

 

            University of Cape Town MB Manual

            Brinkman Primer design Rules

            IDT Primer Analysis Software

            Chang Bioscience Primo Software

 

RNAi Links

            Fire RNAi by feeding pdf

            Sharp RNAi review pdf

 

Topo Cloning

            Topo Manual