Genetic Testimony = &= nbsp; BIO100 Principles of Biolo= gy Spring 2005
1.&n= bsp; Define the following words or phrases (give examples where appropriate):
Alleles
Autoradiograph
Capillary electrophoresis
CODIS
Complementary base pairing
DNA sequence
Gel electrophoresis
Genome
Gene
Genotype
Heterozygous
Homozygous
Hybridization
The Innocence Project
Locus
mtDNA<= /span>
Multiplexing
Non-coding regions or “junk” DNA
Nucleotide
Polymerase chain reaction
Polymorphism
Probe
Restriction enzyme
RFLP
SNP
STR
VNTR
Y Chromosome analysis
2.&n= bsp; Describe the first use of DNA profiling in a crimin= al investigation. In what year w= as this case solved?
3.&n= bsp; Why are VNTRs so useful= in DNA profiling?
4.&n= bsp; Describe the steps to create a VNTR profile.
5.&n= bsp; Describe the steps in polymerase chain reaction.
6.&n= bsp; Describe the steps in STR profiling.
7.&n=
bsp;
What was the method of choice for DNA identificatio=
n of
victims after
8.&n= bsp; When DNA from an evidence sample is compared to the profile from a suspect, what are the three possible outcomes?
9.&n= bsp; If a DNA profile from a suspect and evidence is a m= atch at one locus, is this enough to convict a person? Explain.
10.&= nbsp; How is a profile probability calculated? How many loci are needed to convince you that someone committed a serious crime?
11.&= nbsp; What can forensic DNA profiling be used for?
12.&= nbsp; How reliable is DNA profile technology? What issues can make this technique more or less reliable?
13.&= nbsp; What are the main problems of forensic DNA profiling?
14.&= nbsp; Is it possible for an innocent person to be convicted based on DNA evidence?
15.&= nbsp; Do you think everyone’s DNA profile should be on file? Why or why not?